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CD74 (B-Cell Marker) Antibody [CLIP/813]

In Stock
Catalog Number Formulation Size Price
972-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
972-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
972-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD74 (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Mouse Monoclonal Antibody (CLIP/813).

CD74 (B-Cell Marker) Antibody - Image 2

Immunofluorescent staining of Raji cells. CD74 Mouse Monoclonal Antibody (CLIP/813) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

CD74 (B-Cell Marker) Antibody - Image 3

Flow Cytometric Analysis of Human Raji cells. CD74 Mouse Monoclonal Antibody (CLIP/813;) followed by goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).

CD74 (B-Cell Marker) Antibody - Image 4

Western Blot Analysis of Raji cell lysate using CD74 Mouse Monoclonal Antibody (CLIP/813)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of ~35kDa, identified as CD74. It is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cancer, Cardiovascular, Immunology, B Cell Markers
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Late endosome, Lysosome, trans-Golgi network
Gene Name
CD74
Positive Control
Daudi or Raji cells. Tonsil or lymph node.
Immunogen
Recombinant full-length human CD74 protein
Alternate Names
HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Database Links

Entrez Gene ID
972
SwissProt
P044233

Additional Information

Clone
CLIP/813
Chromosome Location
5q33.1
Mol. Weight of Antigen
33-41kDa

Related Antibodies

View All CD74 Antibodies

Functions

  • Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.
  • Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215).

Key References

  • Wilson, K.M., et al. 1993. Cell-surface expression of human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain (CD74) does not always correlate with cell-surface expression of HLA class II molecules. Immunology 79: 331-335.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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