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CD83 (Marker of Activated Dendritic Cells) Antibody [1H4b]

In Stock
Catalog Number Formulation Size Price
9308-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
9308-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
9308-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD83 Antibody in Immunohistochemistry (IHC (P))

CD83 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD83 Mouse Monoclonal Antibody (1H4b). Inset: PBS instead of primary antibody; secondary only negative control.

CD83 Antibody in SDS-PAGE

CD83 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD93 Mouse Monoclonal Antibody (1H4b). Confirmation of Purity and Integrity of Antibody.

CD83 Antibody in Western Blot (WB)

CD83 Antibody in Western Blot (WB)

Western Blot Analysis of RH-30 and PC-3 lysate using CD83 Mouse Monoclonal Antibody (1H4b).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD83 is a heavily glycosylated membrane protein of the immunoglobulin (Ig) superfamily that is expressed in mature Dendritic cells, Langerhans cells and interdigitating reticulum cells within lymphoid tissues. Structurally, CD83 resembles other Ig superfamily members, which have an extracellular V-type Ig-like domain, a single transmembrane domain and a 40 residue cytoplasmic tail. CD83 expression is used as a marker for mature, antigen presenting Dendritic cells that are capable of generating tumor-specific T cell immunity, a phenotype with implications as an anti-cancer vaccine. CD83-IgG1(Fc) chimera studies indicate that CD83 is a sialic acid-binding, Ig-like Lectin (Siglec) adhesion molecule that is involved in cell adhesion/signaling by hosting Dendritic cell interactions with monocytes and CD8+ T cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immuno Oncology, Immune checkpoint
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Membrane
Gene Name
CD83
Positive Control
Human brain, tonsil, lymph node or spleen, RH3-, PC-3
Immunogen
Prokaryotic recombinant protein corresponding to 124 amino acids of the external N-terminus of the CD83 molecule.
Alternate Names
CD83 antigen, CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15; CD83, BL11; HB15; B-cell activation protein

Database Links

Entrez Gene ID
9308
SwissProt
Q01151

Additional Information

Clone
1H4b
Chromosome Location
6p23
Mol. Weight of Antigen
23kDa

Related Antibodies

View All CD83 Antibodies

Functions

  • May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Key References

  • Batool A, Liu H, Liu YX, Chen SR. CD83, a Novel MAPK Signaling Pathway Interactor.Küppers R. CD83 in Hodgkin lymphoma.Wu Z, Yoshikawa T, Inoue S, et al.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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