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CD86 (Dendritic Cells Maturation Marker) Antibody [BU63]

Name: CD86 (Dendritic Cells Maturation Marker) Antibody
SKU: 942-MSM1-PE-100T
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CD86 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMCs). Red: PE-labeled CD86 Mouse Monoclonal Antibody (BU63); Green: Isotype Control.

CD86 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD86 Mouse Monoclonal Antibody (BU63). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Summary

Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin’s disease.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Functional Studies, Immunofluorescence

Species Reactivity

Human

Research Areas

Cardiovascular, Immunology, B Cell Markers, Cytokine Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Infectious Disease, Signal Transduction

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cell membrane, Cell surface

Gene Name

CD86

Positive Control

293T or Jurkat cells. Monocytes and dendritic cells.

Immunogen

ARH-77 (B-lymphoblastoid cell line)

Alternate Names

T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2

Database Links

Entrez Gene ID

942

SwissProt

P42081

Additional Information

Clone

BU63

Chromosome Location

3q13.33

Mol. Weight of Antigen

70kDa

Functions

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.

Key References

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Mauri D et. al. J Immunol. 1995;155(1):118-27.
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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CD86 (Dendritic Cells Maturation Marker) Antibody [BU63]

CD86 Antibody in Flow Cytometry (FC)

CD86 Antibody in SDS-PAGE

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

Related Antibodies

Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody [rC86/6872]

Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody [rC86/1146]

Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody [C86/6500R]

Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody [C86/2160R]