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CD8A (Cytotoxic- & Suppressor T-Cell Marker) Antibody [C8/468 + C8/144B]

Name: CD8A (Cytotoxic- &amp; Suppressor T-Cell Marker) Antibody
SKU: 925-MSM3
Availability: InStock

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD8 Mouse Monoclonal Antibody (C8/468 + C8/144B).

SDS-PAGE Analysis of Purified CD8 Mouse Monoclonal Antibody (C8/468 + C8/144B). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Immunohistochemistry

Species Reactivity

Human

Research Areas

Cardiovascular, Immuno Oncology, Immunology, Dendritic Cell Marker, Hematopoietic Stem Cells, PD-1 blockade immunotherapy

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cell membrane, Secreted

Gene Name

CD8A

Positive Control

HuT78 or hPBL. Tonsil.

Immunogen

Human CD8 recombinant protein (C8/468); A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule (C8/144B)

Alternate Names

T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain

Database Links

Entrez Gene ID

925

SwissProt

P01732

Additional Information

Clone

C8/468 + C8/144B

Chromosome Location

2p11.2

Mol. Weight of Antigen

32kDa

Functions

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Key References

Mason DY, et. al. Journal of Clinical Pathology, 1992, 45(12):1084-8.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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CD8A (Cytotoxic- & Suppressor T-Cell Marker) Antibody [C8/468 + C8/144B]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

Related Antibodies

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Recombinant CD8A (Cytotoxic / Suppressor T-Cell Marker) Antibody [CD8/7793R]

Recombinant CD8A (Cytotoxic / Suppressor T-Cell Marker) Antibody [CD8/3892R]

Recombinant CD8A (Cytotoxic / Suppressor T-Cell Marker) Antibody [CD8/4391R]

Recombinant CCD8A (Cytotoxic- & Suppressor T-Cell Marker) Antibody [rCD8/9211]

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