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CD8A (Cytotoxic / Suppressor T-Cell Marker) Antibody [RIV11]

In Stock
Catalog Number Formulation Size Price
925-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
925-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
925-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified CD8A Mouse Monoclonal Antibody (RIV11). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD8A Mouse Monoclonal Antibody (RIV11). Confirmation of Integrity and Purity of Antibody.

Immunofluorescence staining of human PBMC cells. CD8A Mouse Monoclonal Antibody (RIV11) followed by goat anti-mouse IgG-CF488 (green).

Immunofluorescence staining of human PBMC cells. CD8A Mouse Monoclonal Antibody (RIV11) followed by goat anti-mouse IgG-CF488 (green).

Flow Cytometric Analysis of human PBMC cells. CD8A Mouse Monoclonal Antibody (RIV11) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Flow Cytometric Analysis of human PBMC cells. CD8A Mouse Monoclonal Antibody (RIV11) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated 伪 and 尾 subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8伪 interacts with the 伪3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the 伪 chain recruits the tyrosine kinase Lck, which is essential for T cell activation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Dendritic Cell Marker, Hematopoietic Stem Cells, PD-1 blockade immunotherapy
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CD8A
Positive Control
HuT78 or hPBL. Human tonsil.
Immunogen
Recombinant full-length human CD8A protein
Alternate Names
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain

Database Links

Entrez Gene ID
925
SwissProt
P01732

Additional Information

Clone
RIV11
Chromosome Location
2p11.2
Mol. Weight of Antigen
32kDa

Related Antibodies

View All CD8A Antibodies

Functions

  • Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Key References

  • Knapp W. et. al. Leukocyte Typing IV, p342-343, Oxford University Press, 1989
  • Parnes JR, CD4 and CD8 in T cell lineage commitment: alterations induced by expression of a CD8/CD4 chimeric transgene. Semin Immunol 1994, 6:221-229.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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