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CD99 / MIC2 (Ewing's Sarcoma Marker) Antibody [SPM586]

In Stock
Catalog Number Formulation Size Price
4267-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4267-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4267-MSM1X-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).

Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Rat
Research Areas
Immunology
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Membrane
Gene Name
CD99
Positive Control
Pancreas or Ewing's sarcoma.
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes
Alternate Names
CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2

Database Links

Entrez Gene ID
4267
SwissProt
P14209

Additional Information

Clone
SPM586
Chromosome Location
Xp22.33
Mol. Weight of Antigen
27-32kDa

Related Antibodies

View All CD99 Antibodies

Functions

  • Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

Key References

  • Sandrin MS, et. al. Immunogenetics, 1992, 35(4):283-5.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein L. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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