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Recombinant CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) Antibody [rCDX2/9241]

In Stock
Catalog Number Formulation Size Price
1045-MSM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1045-MSM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1045-MSM15-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified CDX2 Recombinant Mouse Monoclonal Antibody (rCDX2/9241). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified CDX2 Recombinant Mouse Monoclonal Antibody (rCDX2/9241). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human colon stained with CDX2 Recombinant Mouse Monoclonal Antibody (rCDX2/9241). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon stained with CDX2 Recombinant Mouse Monoclonal Antibody (rCDX2/9241). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids �and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma.However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker, Stem Cell Differentiation
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Nucleus.
Gene Name
CDX2
Positive Control
HT29 cells. Human colon carcinoma
Immunogen
Recombinant fragment (around aa100-300) of human CDX2 protein (exact sequences are proprietary)
Alternate Names
Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2, Caudal type homeobox 2; Caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX2

Database Links

Entrez Gene ID
1045
SwissProt
Q99626

Additional Information

Clone
rCDX2/9241
Chromosome Location
13q12.2
Mol. Weight of Antigen
40kDa

Related Antibodies

View All CDX2 Antibodies

Functions

  • Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).

Key References

  • Mallo, G.V., et al. 1998. Expression of the Cdx1 and Cdx2 homeotic genes leads to reduced malignancy in colon cancer-derived cells. J. Biol. Chem. 273: 14030-14036.
  • Silberg, D.G., et al. 2000. Cdx1 and cdx2 expression during intestinal development. Gastroent. 119:961 971.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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