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CELA3A / Chymotrypsin like elastase 3A Antibody [CELA3A/12553]

In Stock
Catalog Number Formulation Size Price
10136-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10136-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10136-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CELA3A Antibody in Immunohistochemistry (IHC (P))

CELA3A Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human pancreas stained with CELA3A Mouse Monoclonal Antibody (CELA3A/12553). Inset: PBS instead of primary antibody; secondary only negative control.

CELA3A Antibody in SDS-PAGE

CELA3A Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CELA3A Mouse Monoclonal Antibody (CELA3A/12553). Confirmation of Purity and Integrity of Antibody.

CELA3A Antibody in Western Blot (WB)

CELA3A Antibody in Western Blot (WB)

Western Blot Analysis of human pancreas tissue lysate using CELA3A Mouse Monoclonal Antibody (CELA3A/12553).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Elastases belong to a subfamily of serine proteases that function to catalytically hydrolyze a wide variety of proteins. Elastase-3A, also known as ELA3A or ELA3, is a 270 amino acid protein that belongs to the elastase family and acts as an alanine-specific protease. Secreted as a zymogen from the pancreas, Elastase-3A functions as a digestive enzyme in the intestine that participates in metabolic degradation pathways by preferentially cleaving proteins that have alanine residues. In addition, Elastase-3A is thought to play a role in the metabolism and transport of cholesterol molecules in the intestine. Altered expression of Elastase-3A may be associated with pancreatic ductal carcinoma, a highly fatal cancer of the pancreatic ducts

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Extracellular space
Gene Name
CELA3A
Positive Control
Human pancreas or pancreatic cancer.
Immunogen
Recombinant fragment (around aa50-250) of human CELA3A protein (exact sequence is proprietary)
Alternate Names
chymotrypsin-like elastase family member 3A, Chymotrypsin-like elastase family member 3A; Elastase IIIA; Elastase-3A; Protease E; ELA3A; ELA3; CELA3A, ELA3; ELA3A

Database Links

Entrez Gene ID
10136
SwissProt
P09093

Additional Information

Clone
CELA3A/12553
Chromosome Location
1p36.12
Mol. Weight of Antigen
29kDa

Related Antibodies

View All CELA3A Antibodies

Functions

  • Efficient protease with alanine specificity but only little elastolytic activity.

Key References

  • Qiao, X., Qi, X., Xing, P., Liu, T., Hao, H., Yang, X., Jiang, B., Cui, M., & Su, X. (2023). Tandem Mass Tag-Based Proteomic Profiling Identifies Biomarkers in Drainage Fluid for Early Detection of Anastomotic Leakage after Rectal Cancer Resection. Journal of proteome research, 22(11), 3559–3569. https://doi.org/10.1021/acs.jproteome.3c00394
  • Xiao, Y., Zhang, B., Cloyd, J. M., Xu, G., Du, S., Mao, Y., & Pawlik, T. M. (2022). Gene signature and connectivity mapping to assist with drug prediction for pancreatic ductal adenocarcinoma. Surgical oncology, 44, 101849. https://doi.org/10.1016/j.suronc.2022.101849

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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