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Clathrin, Light Chain Antibody [SPM174]

In Stock
Catalog Number Formulation Size Price
1211-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1211-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1211-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).

Formalin-fixed, paraffin-embedded human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, Infectious Disease, Signal Transduction
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Coated pit, Cytoplasm, Cytoplasmic vesicle membrane, Cytoskeleton, Membrane, Spindle
Gene Name
CLTA
Positive Control
HeLa cells. Placenta or Prostate Carcinoma.
Immunogen
Purified recombinant N-terminal fragment of human Clathrin Light Chain
Alternate Names
Clathrin light chain A, Clathrin light chain A; Clathrin light chain B; Clathrin light chain LCA; Clathrin light chain LCB; Clathrin light polypeptide A; Clathrin light polypeptide B; Clathrin, light polypeptide (Lca); Clathrin, light polypeptide (Lcb); CLTA; CLTB

Database Links

Entrez Gene ID
1211; 1212
SwissProt
P09496; P09497

Additional Information

Clone
SPM174
Chromosome Location
9p13.3
Mol. Weight of Antigen
31-44kDa

Related Antibodies

View All CLTA Antibodies

Functions

  • Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).

Key References

  • Jackson, A.P., et al. 1988. Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species. J. Biol. Chem. 263: 16688-16695.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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