Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Collagen IV alpha 5 chain / COL4A5 Antibody [COL4A5/9396R]

In Stock
Catalog Number Formulation Size Price
1287-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1287-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1287-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human pancreas stained with COL4A5 Recombinant Rabbit Monoclonal Antibody (COL4A5/9396R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human pancreas stained with COL4A5 Recombinant Rabbit Monoclonal Antibody (COL4A5/9396R). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified COL4A5 Recombinant Rabbit Monoclonal (COL4A5/9396R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified COL4A5 Recombinant Rabbit Monoclonal (COL4A5/9396R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Type IV collagen is the major structural component of the glomerular basement membrane (GBM), forming a hexagonal network together with lamins, proteoglycans, and nestin. Deletions of the N-terminal regions of COL4A5 and COL4A6 were found in Xq22.3 chromosome deletion syndrome and were located in an end-to-end manner. This results in a phenotype characteristic of diffuse leiomyomatosis and Alport syndrome (DL-ATS).

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Lung Cancer, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted. Basement membrane.
Gene Name
COL4A5
Positive Control
Human placenta or tonsil.
Immunogen
Recombinant fragment (around aa1400-1685) of human COL4A protein (exact sequence is proprietary)
Alternate Names
Collagen alpha-5(IV) chain, Collagen alpha-5(IV) chain; COL4A5

Database Links

Entrez Gene ID
1287
SwissProt
P29400

Additional Information

Clone
COL4A5/9396R
Chromosome Location
Xq22.3
Mol. Weight of Antigen
161kDa

Related Antibodies

View All COL4A5 Antibodies

Functions

  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Key References

  • Tsugio Seki et al. Histochem Cell Biol (1998) 110:359-366.
  • Yoshioka K et al. Am. J. Pathol (1994) 144: 986-996.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Collagen IV alpha 5 chain / COL4A5 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales