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Creatine Phosphokinase-BB (CK-BB) Antibody [CKBB/6567]

In Stock
Catalog Number Formulation Size Price
1152-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1152-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1152-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CKBB Antibody in Western Blot (WB)

CKBB Antibody in Western Blot (WB)

Western blot analysis of Y79 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6567).

Creatine Phosphokinase-BB (CK-BB) Antibody - Image 2

Formalin-fixed, paraffin-embedded human salivary gland stained with CKBB Mouse Monoclonal Antibody (CKBB/6567). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Creatine Phosphokinase-BB (CK-BB) Antibody - Image 3

Formalin-fixed, paraffin-embedded human brain stained with CKBB Mouse Monoclonal Antibody (CKBB/6567). Inset: PBS instead of primary antibody; secondary only negative control.

CKBB Antibody in Western Blot (WB)

CKBB Antibody in Western Blot (WB)

Western blot analysis of HEK293 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6567).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm.
Gene Name
CKB
Positive Control
Y79 or HEK293 cells. Human cerebellum.
Immunogen
Recombinant human full-length CKB protein
Alternate Names
Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, BCK; Brain creatine kinase; Ckb; Creatine kinase B chain, Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine phosphokinase BB; Epididymis luminal protein 211; Epididymis secretory protein Li 29; HEL 211

Database Links

Entrez Gene ID
1152
SwissProt
P12277

Additional Information

Clone
CKBB/6567
Chromosome Location
14q32.32
Mol. Weight of Antigen
42kDa

Related Antibodies

View All CKB Antibodies

Functions

  • Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).

Key References

  • Lowe, M.T., et al. 2013. J. Cereb. Blood Flow Metab. 33: 1295-1306.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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