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Recombinant Cyclin E (G1/S-Phase Cyclin) Antibody [CCNE1/4935R]

In Stock
Catalog Number Formulation Size Price
898-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
898-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
898-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Formalin-fixed, paraffin-embedded human endometrium stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Formalin-fixed, paraffin-embedded human endometrium stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Western Blot Analysis of HCT-116 cell lysate using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Western Blot Analysis of HCT-116 cell lysate using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

SDS-PAGE Analysis of Purified Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R). Confirmation of Purity and Integrity of Antibody.

CCNE1 Antibody in Western Blot (WB)

CCNE1 Antibody in Western Blot (WB)

Western Blot Analysis of HCT-116 cell lysate using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Infectious Disease, Lung Cancer, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
CCNE1
Positive Control
HCT116 or HeLa cells. Human breast or colon carcinoma., Jurkat
Immunogen
Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)
Alternate Names
G1/S-specific cyclin-E1, CCNE1; Cyclin E1; G1/S-specific cyclin-E1

Database Links

Entrez Gene ID
898
SwissProt
P24864

Additional Information

Clone
CCNE1/4935R
Chromosome Location
19q12
Mol. Weight of Antigen
53kDa

Related Antibodies

View All CCNE1 Antibodies

Functions

  • Essential for the control of the cell cycle at the G1/S (start) transition.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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