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CYP2C9 / Cytochrome P450 family 2 subfamily C member 9 Antibody [CYP2C9/13121]

In Stock
Catalog Number Formulation Size Price
1559-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1559-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1559-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CYP2C9 Antibody in Immunohistochemistry (IHC (P))

CYP2C9 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma (HCC) stained with CYP2C9 Mouse Monoclonal Antibody (CYP2C9/13121). Inset: PBS instead of primary antibody; secondary only negative control.

CYP2C9 Antibody in SDS-PAGE

CYP2C9 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CYP2C9 Mouse Monoclonal Antibody (CYP2C9/13121). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Metabolism
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum, Microsome membrane
Gene Name
CYP2C9
Positive Control
Human liver or hepatocellular carcinoma.
Immunogen
Recombinant fragment (around aa1-200) of human CYP2C9 protein (exact sequence is proprietary)
Alternate Names
CYP2C9, Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; PDCD1L2; CD273; B7DC; PDCD1LG2; PDL2, cytochrome P-450 S-mephenytoin 4-hydroxylase; cytochrome P-450MP; cytochrome P450 PB-1; cytochrome P450, family 2, subfamily C, polypeptide 9; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase

Database Links

Entrez Gene ID
1559
SwissProt
P11712

Additional Information

Clone
CYP2C9/13121
Chromosome Location
10q23.33
Mol. Weight of Antigen
55kDa

Related Antibodies

View All CYP2C9 Antibodies

Functions

  • A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids (PubMed:7574697, PubMed:9866708, PubMed:9435160, PubMed:12865317, PubMed:15766564, PubMed:19965576, PubMed:21576599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:7574697, PubMed:9866708, PubMed:9435160, PubMed:12865317, PubMed:15766564, PubMed:19965576, PubMed:21576599). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) (PubMed:7574697, PubMed:15766564, PubMed:19965576, PubMed:9866708). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599). Exhibits low catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed:12865317). Catalyzes bisallylic hydroxylation and hydroxylation with double-bond migration of polyunsaturated fatty acids (PUFA) (PubMed:9866708, PubMed:9435160). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed:11950794). Contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan (PubMed:25994031).

Key References

  • Zhang C, Li Q, Cheng Y, et al. Elfaki I Mir R, Almutairi FM, Duhier FMA.
  • Wang, X., et al. (2019). Identification of prognostic biomarkers for patients with hepatocellular carcinoma after hepatectomy. Oncology reports, 41(3), 1586–1602. https://doi.org/10.3892/or.2019.6953
  • Kuo, S. H., et al. (2017). Polymorphisms of ESR1, UGT1A1, HCN1, MAP3K1 and CYP2B6 are associated with the prognosis of hormone receptor-positive early breast cancer. Oncotarget, 8(13), 20925–20938. https://doi.org/10.18632/oncotarget.14995
  • Elfaki, I., et al. (2018). Cytochrome P450: Polymorphisms and Roles in Cancer, Diabetes and Atherosclerosis. Asian Pacific journal of cancer prevention : APJCP, 19(8), 2057–2070. https://doi.org/10.22034/APJCP.2018.19.8.2057
  • Zhong, X., et al. (2019). Identification of crucial miRNAs and genes in esophageal squamous cell carcinoma by miRNA-mRNA integrated analysis. Medicine, 98(27), e16269. https://doi.org/10.1097/MD.0000000000016269
  • Ren, X., et al. (2018). Downregulation of CYP2A6 and CYP2C8 in Tumor Tissues Is Linked to Worse Overall Survival and Recurrence-Free Survival from Hepatocellular Carcinoma. BioMed research international, 2018, 5859415. https://doi.org/10.1155/2018/5859415

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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