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Recombinant Cystatin A Antibody [CSTA/9087R]

In Stock
Catalog Number Formulation Size Price
1475-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1475-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1475-RBM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cystatin A Antibody

Formalin-fixed, paraffin-embedded human lymph node stained with Cystatin A Recombinant Rabbit Monoclonal Antibody (CSTA/9087R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Cystatin A Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with Cystatin A Recombinant Rabbit Monoclonal Antibody (CSTA/9087R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Cystatin A Antibody - Image 3

SDS-PAGE Analysis of Purified Cystatin A Recombinant Rabbit Monoclonal Antibody (CSTA/9087R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology, Nuclear Marker
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell Surface. Cytoplasm. Nucleus.
Gene Name
CSTA
Positive Control
THP-1 cells. Human tonsil or prostate tissue (IHC).
Immunogen
Recombinant fragment of human CSTA protein (around aa 1-98) (exact sequence is proprietary)
Alternate Names
Cystatin-A | Cystatin-A, N-terminally processed, Cystatin-AS, Stefin-A, STF1; STFA; AREI; cstA; Stefin A

Database Links

Entrez Gene ID
1475
SwissProt
P01040

Additional Information

Clone
CSTA/9087R
Chromosome Location
3Q21.1
Mol. Weight of Antigen
11kDa

Related Antibodies

View All CSTA Antibodies

Functions

  • This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.

Key References

  • Takahashi, H., et al. 2001. J. Biol. Chem. 276: 36632-36638.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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