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Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody [LHK1]

In Stock
Catalog Number Formulation Size Price
3848-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3848-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3848-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 1 Mouse Monoclonal Antibody (LHK1).

Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Cervix stained with Cytokeratin 1 Mouse Monoclonal Antibody (LHK1).

Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified Cytokeratin 1 Mouse Monoclonal Antibody (LHK1). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10). Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human, Rat
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane
Gene Name
KRT1
Positive Control
Skin or Cervix.
Immunogen
A 13-amino acid peptide (VRFVSTSYSGVTR) from the C-terminus of human KRT1 protein
Alternate Names
Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, 67kDa Cytokeratin; CK1; Cytokeratin-1; EHK1; Epidermolytic hyperkeratosis 1; EPPK; K1; Keratin Type II Cytoskeletal 1; Keratin-1; KRT1; KRT1A; NEPPK; Type II cytoskeletal 1; Type-II keratin Kb1

Database Links

Entrez Gene ID
38748
SwissProt
P04264

Additional Information

Clone
LHK1
Chromosome Location
12q13.13
Mol. Weight of Antigen
~67kDa

Related Antibodies

View All KRT1 Antibodies

Functions

  • May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

Key References

  • Bloor et al. 2003. Am J Pathol. 162(3):963-75.
  • Leigh et al. 1993. Br J Dermatol. 129(2):110-9.
  • Waseem et al. 1999. J Invest Dermatol. 112(3):362-9.
  • Machesney et al. 1998. Am J Pathol. 152(5):1133-41.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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