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Cytokeratin 18 (KRT18) Antibody [SPM265]

In Stock
Catalog Number Formulation Size Price
3875-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3875-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3875-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 18 (KRT18) Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 18 Mouse Monoclonal Antibody (SPM265).

Cytokeratin 18 (KRT18) Antibody - Image 2

Western Blot 1) HeLa and 2) A431 lysate using Cytokeratin 18 Mouse Monoclonal Antibody (SPM265).

Cytokeratin 18 (KRT18) Antibody - Image 3

Confocal Immunofluorescence of HeLa cells Cytokeratin 18 Mouse Monoclonal Antibody (SPM265). labeled with CF488 (Green); Reddot is used to label the nuclei.

Cytokeratin 18 (KRT18) Antibody - Image 4

Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (SPM265).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Goat, Human, Pig, Rat
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Nucleolus, Nucleus, Perinuclear region
Gene Name
KRT18
Positive Control
A431 cells. Breast Cancer., HeLa, MCF-7
Immunogen
PMC-42 human breast carcinoma cells
Alternate Names
Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Database Links

Entrez Gene ID
3875
SwissProt
P05783

Additional Information

Clone
SPM265
Chromosome Location
12q13.13
Mol. Weight of Antigen
45kDa

Related Antibodies

View All KRT18 Antibodies

Functions

  • Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Key References

  • Kovarik J et al. Int J Cancer, Supplement. 3:50-55 (1988).
  • Lane EB, Alexander CM. Sem Cancer Biol 1:165-179 (1990).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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