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Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody [KRT5.6/9103]

In Stock
Catalog Number Formulation Size Price
MSM3-9103-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM3-9103-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM3-9103-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/9103). Inset: PBS instead of primary antibody; secondary only negative control.

Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody - Image 2

SDS-PAGE Analysis Purified Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/9103). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cytokeratin 5 is a basic protein that is closely related to cytokeratin 6. They share similar tissue distribution and are found in various proportions in many non-keratinizing stratified squamous epithelia eg. tongue mucosa, as well as in basal epithelia of trachea, basal cells of epidermis, hair follicles, sebaceous and sweat glands of skin, luminal cells of the mammary gland, basal cells of prostate, urothelium, vagina and endocervical mucosa. Cytokeratins 5 and 6 are also expressed in basal cell epitheliomas, squamous cell carcinomas of skin, tongue, epiglottis and of the rectal-anal region. Cytokeratin 5&6 is a useful aid for classification of epithelioid mesotheliomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm.
Gene Name
KRT5
Positive Control
Human prostate or skin tissue.
Immunogen
Recombinant fragments of human Cytokeratin 5 (KRT5) and Cytokeratin 6 proteins (exact sequences are proprietary)
Alternate Names
Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK5; Keratin-5; KRT5; Cytoskeletal 6A; KRT6A

Database Links

Entrez Gene ID
3852 (KRT5); 3853 (KRT6A)
SwissProt
P13647 (KRT5); P02538 (KRT6A)

Additional Information

Clone
KRT5.6/9103
Chromosome Location
12q13.13 (KRT5 and KRT6A);
Mol. Weight of Antigen
58kDa (KRT5); 56kDa (KRT6)

Related Antibodies

View All KRT5 Antibodies

Functions

  • Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).

Key References

  • Ordonez NG1. Value of cytokeratin 5/6 immunostaining in distinguishing epithelial mesothelioma of the pleura from lung adenocarcinoma. Am J Surg Pathol. 1998; 22:1215-1221.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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