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Cytokeratin 5/8 Antibody [SPM268]

In Stock
Catalog Number Formulation Size Price
MSM13X-948-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM13X-948-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM13X-948-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 5/8 Antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (SPM268).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep
Isotype / Light Chain
IgG1 / Kappa
Gene Name
KRT5
Positive Control
HepG2 or A431 cells. Skin or Squamous cell carcinoma.
Immunogen
Cytoskeletal preparation from HeLa cells
Alternate Names
Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK5; DDD; EBS2; Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; K5; Keratin complex 2, basic, Gene 5; Keratin Type II Cytoskeletal 5; Keratin-5; KRT5; KRT5A; Type II Cytoskeletal 5; Type-II Keratin Kb5. CK8; CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8

Database Links

Entrez Gene ID
3852; 3856
SwissProt
P13647 ; P05787

Additional Information

Clone
SPM268
Chromosome Location
12q13.13 (CK5) & 17q21.2 (CK8)
Mol. Weight of Antigen
58kDa (CK5); 52.5kDa (CK8)

Related Antibodies

View All KRT5 Antibodies

Key References

  • Bartkova et al. Neoplasma 38(4), 439-46 (1991).
  • Vojtek et al. Folia Biol. 37(3-4): 197-206 (1991).
  • Bartek et al. J. of Pathology 164, 215-24 (1991).
  • Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).

PubMed Links

Storage & Stability

Store at 2 to 8°C. Antibody is stable for 24 months.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

0.5 ml at 200 �g/ml of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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