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Printable Datasheet

Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody [KRT5/6399R]

In Stock
Catalog Number Formulation Size Price
3852-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3852-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3852-RBM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody (KRT5/6399R).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody (KRT5/6399R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KRT5/6399R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KRT5/6399R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This Mab recognizes a protein of 58kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, yoepithelial, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology, Basal Cell Marker
Isotype / Light Chain
IgG / Kappa
Gene Name
KRT5
Positive Control
esophagus or bladder tissue (IHC)., Human tonsil
Immunogen
Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)
Alternate Names
Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, 58kDa Cytokeratin; DDD1; Epidermolysis Bullosa Simplex 2 (EBS2); Keratin 5; Keratin, Type II Cytoskeletal 5; Keratin-5; Type-II Cytoskeletal 5; Type-II keratin Kb5

Database Links

Entrez Gene ID
3852
SwissProt
P13647

Additional Information

Clone
KRT5/6399R
Chromosome Location
12q13.13
Mol. Weight of Antigen
58kDa

Related Antibodies

View All KRT5 Antibodies

Key References

  • Ordonez NG. Human Pathology. 2007; 38:1 16.

PubMed Links

Storage & Stability

Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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