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Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [KRT7/4387R]

In Stock
Catalog Number Formulation Size Price
3855-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3855-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3855-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embeddedhuman bladdercarcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Formalin-fixed, paraffin-embeddedhuman bladdercarcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/4387R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KRT7/4387R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KRT7/4387R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cytokeratin 7 in humans is a protein encoded by the KRT7 gene. It is always as a keratin pair with Cytokeratin 19. Cytokeratin 7 is expressed on most ductal and glandular epithelium including lung, breast, bladder and female genital tract, but not in most gastrointestinal epithelium, prostate, hepatocyte and squamous epithelium. In tumor tissues, its expression is absent in colon cancer, prostate cancer and squamous carcinomas. Cytokeratin 7 is often used in concert with Cytokeratin 20 and CDX-2 to aid in distinguishing ovarian, pulmonary and breast carcinomas (CK7+) from colon carcinomas (CK7-).

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT7
Positive Control
Carcinoma of ovary, Cervix, Lung, or breast.
Immunogen
Recombinant fragment (around aa1-100) of human KRT7 protein (exact sequence is proprietary)
Alternate Names
Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Database Links

Entrez Gene ID
3855
SwissProt
P08729

Additional Information

Clone
KRT7/4387R
Chromosome Location
12q13.13
Mol. Weight of Antigen
55kDa

Related Antibodies

View All KRT7 Antibodies

Functions

  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Key References

  • Moll R, et al. Histochem Cell Biol. 2008 Jun;129(6):705-33.
  • Moll R, et al. Cell. 1982 Nov;31(1):11-24.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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