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Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [KRT7/903]

In Stock
Catalog Number Formulation Size Price
3855-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3855-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3855-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903)

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903)

Purified Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903). Confirmation of Integrity and Purity of Antibody.

Purified Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903). Confirmation of Integrity and Purity of Antibody.

Flow Cytometric Analysis of trypsinized methanol-fixed HeLa cells using Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of trypsinized methanol-fixed HeLa cells using Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/903) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT7
Positive Control
A431, Breast cancer., HeLa cells
Immunogen
Recombinant full-length human KRT7 protein
Alternate Names
Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK7; K2C7; K7; Keratin 55k Type II Cytoskeletal; Keratin 7; Keratin Simple Epithelial Type 1 k7; Keratin Type II Cytoskeletal 7; Keratin, 55K Type II Cytoskeletal; Krt2-7; KRT7; Sarcolectin; SCL; Type ii Mesothelial Keratin k7; Type-II Keratin Kb7

Database Links

Entrez Gene ID
3855
SwissProt
P08729

Additional Information

Clone
KRT7/903
Chromosome Location
12q13.13
Mol. Weight of Antigen
54kDa

Related Antibodies

View All KRT7 Antibodies

Functions

  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Key References

  • Fuchs, E. 1995. Keratins and the skin. Annu. Rev. Cell Dev. Biol. 11:123-153.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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