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Cytokeratin 8 (KRT8) Antibody [H1]

In Stock
Catalog Number Formulation Size Price
3856-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3856-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3856-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (‘ring-like, perinuclear’) from ductal (‘peripheral-predominant’) carcinoma of the breast.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Nucleoplasm, Nucleus, Nucleus matrix
Gene Name
Positive Control
A-431 cells. Skin, Colon, HCT-116, HeLa, lung or breast carcinoma., MCF7
Immunogen
Cytoskeleton preparation containing cytokeratin 8
Alternate Names
Keratin, type II cytoskeletal 8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; KRT8; Type-II Keratin Kb8

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
H1
Chromosome Location
12q13.13
Mol. Weight of Antigen
52.5kDa

Functions

  • Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Key References

  • Guelstein VI et. al. Int J Cancer 42:147-53 (1988).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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