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Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody [AE-1]

In Stock
Best Seller
Catalog Number Formulation Size Price
MSM4-253-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM4-253-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM4-253-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, LMW Mouse Monoclonal Antibody (AE-1).

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, LMW Mouse Monoclonal Antibody (AE-1).

Western Blot Analysis of A431 cells using Cytokeratin, LMW Mouse Monoclonal Antibody (AE-1).

Western Blot Analysis of A431 cells using Cytokeratin, LMW Mouse Monoclonal Antibody (AE-1).

SDS-PAGE Analysis of Purified Cytokeratin, LMW Monoclonal Antibody (AE-1). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Cytokeratin, LMW Monoclonal Antibody (AE-1). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat, Turtle
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Gene Name
KRT77
Positive Control
Skin or squamous cell carcinoma (IHC).
Immunogen
Human epidermal keratin
Alternate Names
Keratin, type II cytoskeletal 1b, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

Database Links

Entrez Gene ID
374454
SwissProt
Q7Z794

Additional Information

Clone
AE-1
Chromosome Location
17q21.2
Mol. Weight of Antigen
40-56.5kDa

Related Antibodies

View All KRT77 Antibodies

Key References

  • Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  • Tseng SCG et. al. Cell 1982; 30361.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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