Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody [KRTL/1577R]

In Stock
Catalog Number Formulation Size Price
RBM21-1577-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
RBM21-1577-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
RBM21-1577-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CK LMW Rabbit Recombinant Monoclonal Antibody (KRTL/1577R).

Recombinant Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CK LMW Rabbit Recombinant Monoclonal Antibody (KRTL/1577R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Dog, Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG / Kappa
Gene Name
KRT77
Positive Control
Skin, Squamous cell carcinoma (SCC).
Immunogen
Recombinant human KRT77 protein fragment
Alternate Names
Keratin, type II cytoskeletal 1b, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, K1B; KRT1B; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B

Database Links

Entrez Gene ID
374454
SwissProt
Q7Z794

Additional Information

Clone
KRTL/1577R
Chromosome Location
17q21.2
Mol. Weight of Antigen
56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)

Related Antibodies

View All KRT77 Antibodies

Key References

  • Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  • Tseng SCG et. al. Cell 1982; 30361.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales