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Recombinant Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody [rKRTH/8883]

In Stock
Catalog Number Formulation Size Price
MSM58-8883-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM58-8883-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM58-8883-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, HMW Recombinant Mouse Monoclonal Antibody (rKRTH/8883). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CK HMW Recombinant Mouse Monoclonal Antibody (rKRTH/8883). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm.
Gene Name
KRT76
Positive Control
Epithelial cells|Skin or Adenocarcinomas
Immunogen
Recombinant full-length human KRT76 protein
Alternate Names
Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9

Database Links

Entrez Gene ID
51350
SwissProt
Q01546

Additional Information

Clone
rKRTH/8883
Chromosome Location
12q13.13
Mol. Weight of Antigen
52-67kDa

Related Antibodies

View All KRT76 Antibodies

Functions

  • Probably contributes to terminal cornification.

Key References

  • Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
  • Tseng SCG et. al. Cell 1982; 30361.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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