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Cytokeratin (CAM5.2) (Epithelial Marker) Antibody [CAM5.2]

In Stock
Catalog Number Formulation Size Price
MSM62-9707-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM62-9707-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM62-9707-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin Antibody in Immunohistochemistry (IHC (P))

Cytokeratin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Cytokeratin Mouse Monoclonal Antibody (CAM5.2). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cytokeratin CAM5.2 antibody has a primary reactivity with human keratin proteins that correspond to Moll’s peptides #7 and #8, MW 48 and 52 kDa, respectively. Cytokeratin 7 and 8 are present in secretory epithelia of normal human tissue but not on stratified squamous epithelium.

Cytokeratin LMW CAM5.2 antibody stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas. Anti-Cytokeratin (CAM 5.2) may not react with some squamous cell carcinomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm
Positive Control
Human colon, lung, prostate or breast.
Immunogen
Hybridization of mouse P3/NS-1/1-Ag4-1myeloma cells with spleen cells from BALB/c mice immunized with the human colorectal carcinoma cell line HT29.
Alternate Names
Keratin, type II cytoskeletal 1b, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, K1B; KRT1B; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B

Database Links

Entrez Gene ID
Not Known
SwissProt
N/A

Additional Information

Clone
CAM5.2

Related Antibodies

Key References

  • Albers et al. Am J Pathol 90: 17, 1988.
  • Ellis et al. J Clin Pathol 41: 1064, 1988. Oikawa et al. Biochem
  • Biophys Res Commun 144: 634, 1987.
  • Pfaltz et al. Virchows Arch A 411: 387, 1987.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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