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Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody [KRT/1877R]

In Stock
Best Seller
Catalog Number Formulation Size Price
RBM37-1877-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
RBM37-1877-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
RBM37-1877-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).

Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified Multi-Cytokeratin Rabbit Monoclonal Antibody (KRT/1877R). Confirmation of Integrity and Purity of Antibody.

Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody - Image 3

Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Frog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig, Rat
Isotype / Light Chain
IgG / Kappa
Gene Name
KRT6A
Positive Control
A431 cells, Colon carcinoma., Skin
Immunogen
Keratin-enriched preparation from cultured human epithelial cells
Alternate Names
Keratin, type II cytoskeletal 6A, Cytokeratin-6A, Cytokeratin-6D, Keratin-6A, Type-II keratin Kb6

Database Links

Entrez Gene ID
3851; 3852; 3853; 3854; 286887; 3856; 3858; 3860; 3875
SwissProt
P02538 ; P04259 ; P13647 ; P19013 ; P48668

Additional Information

Clone
KRT/1877R
Mol. Weight of Antigen
Multiple

Related Antibodies

View All KRT6A Antibodies

Functions

  • Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

Key References

  • Bartek J et. al. J Pathol, 1991, 164(3):215-24.
  • Kasper M. Histochemistry, 1991, 95(6):613-20.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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