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Recombinant Cytokeratin, Type I (Epithelial Marker) Antibody [KRTL/7784R]

In Stock
Catalog Number Formulation Size Price
RBM52-7784-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
RBM52-7784-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
RBM52-7784-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin, Type I (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CK Type I Recombinant Rabbit Monoclonal Antibody (KRTL/7784R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation.This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG /
Cellular Localization
Cytoplasm.
Gene Name
KRT77
Positive Control
skin or squamous cell carcinoma.
Immunogen
Synthetic peptide corresponding to Type I Cytokeratins residues within aa100-200 of Type I Cytokeratins
Alternate Names
Keratin, type II cytoskeletal 1b, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, K1B; KRT1B; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B

Database Links

Entrez Gene ID
374454
SwissProt
Q7Z794--------

Additional Information

Clone
KRTL/7784R
Chromosome Location
17q21.2
Mol. Weight of Antigen
60/53/51/45/40kDa

Related Antibodies

View All KRT77 Antibodies

Key References

  • Spagnolo DV, et al. Am J Clin Pathol. 1985 Dec;84(6):697-704.
  • Eichner R, et al. J Cell Biol. 1984 Apr;98(4):1388-96.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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