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DAXX Antibody [PCRP-DAXX-8C2]

In Stock
Catalog Number Formulation Size Price
1616-MSM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1616-MSM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1616-MSM15-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
DAXX Antibody in Immunohistochemistry (IHC (P))

DAXX Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human kidney stained with DAXX Mouse Monoclonal Antibody (PCRP-DAXX-8C2).

DAXX Antibody in Immunofluorescence (IF)

DAXX Antibody in Immunofluorescence (IF)

Immunofluorescence Analysis of PFA-fixed HeLa cells using DAXX Mouse Monoclonal Antibody (PCRP-DAXX-8C2) followed by goat anti-mouse IgG-CF488 (green).

DAXX Antibody in SDS-PAGE

DAXX Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified DAXX Mouse Monoclonal Antibody (PCRP-DAXX-8C2). Confirmation of Purity and Integrity of Antibody.

DAXX Antibody in Flow Cytometry (FC)

DAXX Antibody in Flow Cytometry (FC)

Flow cytometric analysis of PFA-fixed HeLa cells. DAXX Mouse Monoclonal Antibody (PCRP-DAXX-8C2) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Infectious Disease, MAPK Signaling, Transcription Factors
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Centromere, Chromosome, Cytoplasm, Nucleolus, Nucleoplasm, Nucleus, PML body
Gene Name
DAXX
Positive Control
HeLa or U87 cells. Human kidney.
Immunogen
Recombinant full-length human DAXX protein
Alternate Names
Death domain-associated protein 6, Daxx, ETS1-associated protein 1, Fas death domain-associated protein, DAP6; EAP1; BING2; SMIM40

Database Links

Entrez Gene ID
1616
SwissProt
Q9UER7

Additional Information

Clone
PCRP-DAXX-8C2
Chromosome Location
6p21.32
Mol. Weight of Antigen
81.37kDa

Related Antibodies

View All DAXX Antibodies

Functions

  • Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV). Plays a role as a positive regulator of the heat shock transcription factor HSF1 activity during the stress protein response (PubMed:15016915).

Key References

  • Zhong, S., et al. 2000. Role of SUMO-1-modified PML in nuclear body formation. Blood 95: 2748-2752.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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