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DAZL (Deleted in Azoospermia-like) Antibody [DAZL/4253]

In Stock
Catalog Number Formulation Size Price
1618-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1618-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1618-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
DAZL (Deleted in Azoospermia-like) Antibody

SDS-PAGE Analysis of Purified DAZL Mouse Monoclonal Antibody (DAZL/4253) Confirmation of Integrity and Purity of Antibody.

DAZL (Deleted in Azoospermia-like) Antibody - Image 2

Western blot Analysis testis tissue lysate using DAZL Mouse Monoclonal Antibody (DAZL/4253).

DAZL (Deleted in Azoospermia-like) Antibody - Image 3

Formalin-fixed, paraffin-embedded human testis stained with DAZL Mouse Monoclonal Antibody (DAZL/4253).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cynomolgus Monkey, Human, Mouse, Rat
Isotype / Light Chain
IgG1 /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
DAZL
Positive Control
Human testis or ovary tissue.
Immunogen
Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV.
Alternate Names
Deleted in azoospermia-like, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, Deleted in azoospermia-like, DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, DAZH, DAZL1, DAZLA, SPGYLA

Database Links

Entrez Gene ID
1618
SwissProt
Q92904

Additional Information

Clone
DAZL/4253
Chromosome Location
3p24.3
Mol. Weight of Antigen
33kDa

Related Antibodies

View All DAZL Antibodies

Functions

  • RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts (By similarity).

Key References

  • Anderson, R.A., et al. (2007). Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis. BMC Developmental Biology, 7, 136.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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