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SDS-PAGE Analysis of Purified DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6). Confirmation of Purity and Integrity of Antibody.
Flow cytometric analysis of PFA-fixed HeLa cells. DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Immunofluorescent staining of PFA-fixed human cell line MCF7. DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (green); phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3'exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay
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