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Desmoglein-3 (Squamous Cell Marker) Antibody [DSG3/1535]

In Stock
Catalog Number Formulation Size Price
1830-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1830-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1830-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Lung Squamous Cell Carcinoma stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).

Formalin-fixed, paraffin-embedded human Lung Squamous Cell Carcinoma stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cell membrane, Desmosome
Gene Name
DSG3
Positive Control
A431 cells. Skin or Lung Squamous Cell Carcinoma (SCC).
Immunogen
Recombinant human DSG3 extracellular protein fragment
Alternate Names
Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, 130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Database Links

Entrez Gene ID
1830
SwissProt
P32926

Additional Information

Clone
DSG3/1535
Chromosome Location
18q12.1
Mol. Weight of Antigen
130kDa

Related Antibodies

View All DSG3 Antibodies

Functions

  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Key References

  • Savci-Heijink CD, et al. The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung. Am J Pathology 2009; 174(5):1629-37.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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