Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Desmoglein-3 (Squamous Cell Marker) Antibody [DSG3/8251R]

In Stock
Catalog Number Formulation Size Price
1830-RBM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1830-RBM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1830-RBM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified DSG3 Recombinant Rabbit Monoclonal Antibody (DSG3/8251R). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified DSG3 Recombinant Rabbit Monoclonal Antibody (DSG3/8251R). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human skin stained with Desmoglein-3 Recombinant Rabbit Monoclonal Antibody (DSG3/8251R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human skin stained with Desmoglein-3 Recombinant Rabbit Monoclonal Antibody (DSG3/8251R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface
Gene Name
DSG3
Positive Control
A431 cells. Lung squamous cell carcinoma.
Immunogen
Recombinant fragment (around aa300-500) of human DSG3 protein (exact sequence is proprietary)
Alternate Names
Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, 130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Database Links

Entrez Gene ID
1830
SwissProt
P32926Human

Additional Information

Clone
DSG3/8251R
Chromosome Location
18q12.1
Mol. Weight of Antigen
130kDa

Related Antibodies

View All DSG3 Antibodies

Functions

  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Key References

  • Mahoney, MG; Hu, Y; Brennan, D; Bazzi, H; Christiano, AM; Wahl, JK III. Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol. 2006, 15(2):101-9.
  • Wahl JK III. Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21(1):37-44.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Desmoglein-3 (Squamous Cell Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales