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EGFR (Epidermal Growth Factor Receptor) Antibody [GFR450]

In Stock
Catalog Number Formulation Size Price
1956-MSM1-CF488-100T
Purified Ab conjugated to CF488
0.5ml at 100ug/ml
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
EGFR Antibody in Flow Cytometry (FC)

EGFR Antibody in Flow Cytometry (FC)

Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled EGFR Monoclonal Antibody (GFR450).

EGFR Antibody in Immunofluorescence (IF)

EGFR Antibody in Immunofluorescence (IF)

Confocal Immunofluorescent analysis of A431 cells using CF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).

EGFR Antibody in Immunofluorescence (IF)

EGFR Antibody in Immunofluorescence (IF)

Confocal Immunofluorescent analysis of A431 cells using CF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Autophagy, Breast Cancer, Cancer, Cardiovascular, Developmental Biology, AKT Signaling, Bladder Cancer, Colon Cancer, Infectious Disease, MAPK Signaling, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell surface
Gene Name
EGFR
Positive Control
A431 cells. Human breast or bladder cancer.
Immunogen
Recombinant extracellular domain of human EGFR protein
Alternate Names
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5

Database Links

Entrez Gene ID
1956
SwissProt
P00533

Additional Information

Clone
GFR450
Chromosome Location
7p11.2
Mol. Weight of Antigen
~170kDa (wild type) and ~145kDa (vIII variant)

Related Antibodies

View All EGFR Antibodies

Functions

  • Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704, PubMed:17909029). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).
  • Isoform 2 may act as an antagonist of EGF action.
  • (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

Key References

  • Tungekar MF et. al. Journal of Clinical Pathology. 51: 583-587 (1998).

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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