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Recombinant EGFRvIII (Epidermal Growth Factor Receptor, Variant III) Antibody [GFR/2600R]

In Stock
Catalog Number Formulation Size Price
1956-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1956-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1956-RBM13-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Lung SqCC stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).

Formalin-fixed, paraffin-embedded human Lung SqCC stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).

Formalin-fixed, paraffin-embedded human Placenta stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).

Formalin-fixed, paraffin-embedded human Placenta stained with EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R).

SDS-PAGE Analysis of Purified EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified EGFRvIII Rabbit Recombinant Monoclonal Antibody (GFR/2600R). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Autophagy, Breast Cancer, Cancer, Cardiovascular, Developmental Biology, AKT Signaling, Bladder Cancer, Colon Cancer, Infectious Disease, MAPK Signaling, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus, Nucleus membrane, Secreted
Gene Name
EGFR
Positive Control
A431 cells. Placenta, Breast, Colon or Bladder Carcinoma
Immunogen
KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC)
Alternate Names
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, Erbb1 variant III; ERBB1 variant III; HER1 variant III; Proto-oncogene c-ErbB-1 variant III; Receptor Tyrosine Protein Kinase variant III; ErbB1 variant III

Database Links

Entrez Gene ID
1956
SwissProt
P00533

Additional Information

Clone
GFR/2600R
Chromosome Location
7p11.2
Mol. Weight of Antigen
~145kDa (vIII variant)

Related Antibodies

View All EGFR Antibodies

Functions

  • Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704, PubMed:17909029). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity).
  • Isoform 2 may act as an antagonist of EGF action.
  • (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

Key References

  • Wikstrand CJ, et al. Cancer Res. 1995 Jul 15;55(14):3140-8.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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