Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody [EGP40/826]

In Stock
Catalog Number Formulation Size Price
4072-MSM6-CF488-100T
Purified Ab conjugated to CF488
0.5ml at 100ug/ml
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
EpCAM Antibody in Immunofluorescence (IF)

EpCAM Antibody in Immunofluorescence (IF)

Confocal immunofluorescent analysis of SK-OV-3 cells using CF488-labeled EpCAM Mouse Monoclonal Antibody (EGP40/826) (green). DyLight 554 Phalloidin-labeled F-actin filaments (Red). DAPI stained nuclei (blue).

Isotype Control Antibody in Immunofluorescence (IF)

Isotype Control Antibody in Immunofluorescence (IF)

Confocal Immunofluorescent analysis of SK-OV-3 cells using CF488-labeled Isotype Control Mouse Monoclonal Antibody IgG1-(Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody - Image 3

SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (EGP40/826). Confirmation of Purity and Integrity of Antibody.

EpCAM Antibody in Flow Cytometry (FC)

EpCAM Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of PFA fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (EGP40/826) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Stem Cell Differentiation
Isotype / Light Chain
IgG1 /
Cellular Localization
Cell junction, Cell surface, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
MCF-7, HT29 or SK-OV-3 cells (FACS/IF). Breast carcinoma (IHC).
Immunogen
Recombinant fragment (around aa 20-65) from the N-terminus of human TACSTD1/EpCAM protein (exact sequence is proprietary)
Alternate Names
Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Database Links

Entrez Gene ID
4072
SwissProt
P16422

Additional Information

Clone
EGP40/826
Chromosome Location
2q21
Mol. Weight of Antigen
40-43kDa

Related Antibodies

View All EPCAM Antibodies

Functions

  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Key References

  • Bjork, P., et al. 1993. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glyco- protein recognized by the C215 Mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales