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EpCAM / CD326 (Epithelial Marker) Antibody [Cocktail PAN-EpCAM]

In Stock
Catalog Number Formulation Size Price
4072-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4072-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4072-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

Western Blot analysis of human lung tissue lysate using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

Western Blot analysis of human lung tissue lysate using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

SDS-PAGE Analysis of Purified EpCAM Mouse Monoclonal Antibody (PAN-EpCAM). Confirmation of Integrity and Purity of Antibody

SDS-PAGE Analysis of Purified EpCAM Mouse Monoclonal Antibody (PAN-EpCAM). Confirmation of Integrity and Purity of Antibody

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM). It is a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or EpCAM. EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
MCF-7 or HT29 cells. Human breast tumor.
Immunogen
Recombinant human EpCAM protein (full-length and fragments)
Alternate Names
Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1

Database Links

Entrez Gene ID
4072
SwissProt
P16422

Additional Information

Clone
Cocktail PAN-EpCAM
Chromosome Location
2q21
Mol. Weight of Antigen
40-43kDa

Related Antibodies

View All EPCAM Antibodies

Functions

  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Abs purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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