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ER-beta (Estrogen Receptor beta-2) Antibody [57/3]

In Stock
Catalog Number Formulation Size Price
2100-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2100-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2100-MSM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Estrogen Receptor beta 1 Antibody in SDS-PAGE.

Estrogen Receptor beta 1 Antibody in SDS-PAGE.

SDS-PAGE Analysis of Purified Estrogen Receptor beta2 Mouse Monoclonal Antibody (57/3). Confirmation of Integrity and Purity of Antibody.

Estrogen Receptor beta 1 Antibody in Immunohistochemistry (IHC (P)).

Estrogen Receptor beta 1 Antibody in Immunohistochemistry (IHC (P)).

Formalin-fixed, paraffin-embedded human testis stained with ER-beta2 Mouse Monoclonal Antibody (57/3).

Estrogen Receptor beta 1 Antibody in Flow Cytometry (FC).

Estrogen Receptor beta 1 Antibody in Flow Cytometry (FC).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ER-beta2 Mouse Monoclonal Antibody (57/3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, Infectious Disease, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
ESR2
Positive Control
MCF-7 cells. Human testis tissue.
Immunogen
A synthetic peptide (CGMKMETLLPEATMEQ ) from the C terminus of the human estrogen receptor beta 2 isomer
Alternate Names
Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, Estrogen Receptor 2; Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2); Oestrogen Receptor Beta; Estrogen Receptor Beta; ER-BETA; ESTRB

Database Links

Entrez Gene ID
2100
SwissProt
Q92731

Additional Information

Clone
57/3
Chromosome Location
14q23.2
Mol. Weight of Antigen
59kDa

Related Antibodies

View All ESR2 Antibodies

Functions

  • Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).
  • Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Key References

  • Collins, F., et al. (2009). Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα. BMC Cancer, 9, 330.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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