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ERCC1 / RAD10 (Tumor Progression Marker) Antibody [ERCC1/7596]

In Stock
Catalog Number Formulation Size Price
2067-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2067-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2067-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
ERCC1 / RAD10 (Tumor Progression Marker) Antibody

Formalin-fixed, paraffin-embedded human prostate stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/7596). Inset: PBS instead of primary antibody; secondary only negative control.

ERCC1 / RAD10 (Tumor Progression Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human thyroid stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/7596). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

ERCC1 / RAD10 (Tumor Progression Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified ERCC1 Mouse Monoclonal Antibody (ERCC1/7596). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 110kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Nucleus.
Gene Name
ERCC1
Positive Control
HeLa or HepG2 cells. Tonsil or Prostate.
Immunogen
Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)
Alternate Names
DNA excision repair protein ERCC-1, COFS4; DNA excision repair protein ERCC1; ERCC1; Excision repair cross complementing 1; RAD10; UV20

Database Links

Entrez Gene ID
2067
SwissProt
P07992Human

Additional Information

Clone
ERCC1/7596
Chromosome Location
19q13.32
Mol. Weight of Antigen
110kDa

Related Antibodies

View All ERCC1 Antibodies

Functions

  • Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
  • Not functional in the nucleotide excision repair pathway.
  • Not functional in the nucleotide excision repair pathway.
  • Not functional in the nucleotide excision repair pathway.

Key References

  • Miura M, et al. Exp Cell Res 1996;226:126-132.
  • Hayashi T, et al. Mutat Res 1998;407:269-276.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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