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Recombinant Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody [ESR1/6983R]

In Stock
Catalog Number Formulation Size Price
2099-RBM43-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2099-RBM43-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2099-RBM43-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin fixed paraffin embedded human breast carcinoma stained with ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/6983R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin fixed paraffin embedded human breast carcinoma stained with ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/6983R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Western Blot Analysis of human MCF-7 cell lysate using Estrogen Receptor, alpha Rabbit Recombinant MAb (ESR1/6983R).

Western Blot Analysis of human MCF-7 cell lysate using Estrogen Receptor, alpha Rabbit Recombinant MAb (ESR1/6983R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. The receptor molecule has three domains, i.e. the central DNA-binding domain, the hormone-binding domain at the C-terminal, and the transcription-activating domain at the N-terminal. ER mediates regulatory functions of female sex steroids, mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems. Studies have shown ER 伪 is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas. Studies with immunohistochemical assay show that positive steroid hormone status has predicted favorable overall, survival, independently of hormonal treatment. Secondly, ER 伪 can be used as a tumor marker, preferentially in combination with an antibody to progesterone receptor, e.g., in the classification of adenocarcinomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, Infectious Disease, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Cytoplasm, Golgi apparatus, Nucleus
Gene Name
ESR1
Positive Control
MCF-7 cells. Human breast or endometrial carcinoma.
Immunogen
Recombinant fragment (around aa495-595) of human ER alpha protein (exact sequence is proprietary)
Alternate Names
Estrogen receptor, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, Estrogen Receptor alpha delta 4*5,6,7*/654 isoform; Estrogen Receptor alpha delta 4 +49 isoform; Nuclear receptor subfamily 3 group A member 1

Database Links

Entrez Gene ID
2099
SwissProt
P03372

Additional Information

Clone
ESR1/6983R
Chromosome Location
6q25.1
Mol. Weight of Antigen
~67kDa

Related Antibodies

View All ESR1 Antibodies

Functions

  • Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032).
  • Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861).

Key References

  • Zafrani B, et. al. Histopathology 2000; 37(6), 536 545.
  • Harvey JM, et. al. Journal of Clinical Oncology 1999; 17(5), 1474 1481.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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