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Printable Datasheet

Fibronectin Antibody [SPM246]

In Stock
Catalog Number Formulation Size Price
2335-MSM2X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2335-MSM2X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2335-MSM2X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Pancreatic Adenocarcinoma stained with Fibronectin Monoclonal Antibody (SPM246).

Formalin-fixed, paraffin-embedded human Pancreatic Adenocarcinoma stained with Fibronectin Monoclonal Antibody (SPM246).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. This MAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this MAb localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating MAb indicating that it can be used to specifically target tumor vessels in vivo. TV-1 is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or MAbs.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Mouse, Pig, Rat
Research Areas
Cardiovascular, Immunology, Articular Cartilage Extracellular Matrix, Cytokine Signaling, Infectious Disease, Lung Cancer, Mesenchymal Stem Cell Differentiation, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
Fn1
Positive Control
SW156 cells. Kidney.
Immunogen
T-cell lymphoma biopsy
Alternate Names
Fibronectin, Cold-insoluble globulin, Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

Database Links

Entrez Gene ID
2335
SwissProt
P02751

Additional Information

Clone
SPM246
Chromosome Location
2q35
Mol. Weight of Antigen
220kDa (monomer); 440kDa (dimer)

Related Antibodies

View All Fn1 Antibodies

Functions

  • Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).
  • Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Key References

  • Epstein AL, et. al. Cancer Research, 1995, 55(12):2673-80.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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