Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Filaggrin (Keratinocyte Differentiation Marker) Antibody [rFLG/1945]

In Stock
Catalog Number Formulation Size Price
2312-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2312-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2312-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human skin stained with Filaggrin Recombinant Mouse Monoclonal Antibody (rFLG/1945).

Formalin-fixed, paraffin-embedded human skin stained with Filaggrin Recombinant Mouse Monoclonal Antibody (rFLG/1945).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasmic granule
Gene Name
FLG
Positive Control
U-251-MGor A-431 cells. Human skin.
Immunogen
Recombinant human Filaggrin protein fragment (around aa 198-288) (exact sequence is proprietary)
Alternate Names
Filaggrin, ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin

Database Links

Entrez Gene ID
2312
SwissProt
P20930

Additional Information

Clone
rFLG/1945
Chromosome Location
1q21.3
Mol. Weight of Antigen
26-45kDa (Processed); 350kDa (Profilaggrin)

Related Antibodies

View All FLG Antibodies

Functions

  • Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Key References

  • Gan, S.Q., et al. 1990. Biochemistry 29: 9432-9440.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Filaggrin (Keratinocyte Differentiation Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales