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Recombinant GATA-3 (Breast and Urothelial Marker) Antibody [GATA3/4550R]

In Stock
Catalog Number Formulation Size Price
2625-RBM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2625-RBM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2625-RBM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GATA-3 Recombinant Rabbit Monoclonal Antibody (GATA3/4550R).

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GATA-3 Recombinant Rabbit Monoclonal Antibody (GATA3/4550R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using GATA3/4550R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using GATA3/4550R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Western blot analysis of MCF-7 cell lysate using GATA-3 Recombinant Rabbit Monoclonal Antibody (GATA3/4550R). Specific bands were detected for GATA-3 full length (FL) at approx. 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated).

Western blot analysis of MCF-7 cell lysate using GATA-3 Recombinant Rabbit Monoclonal Antibody (GATA3/4550R). Specific bands were detected for GATA-3 full length (FL) at approx. 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human, Rat
Research Areas
Cardiovascular, Immunology, Cytokine Signaling, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
GATA3
Positive Control
Human breast or urothelial carcinoma tissue.
Immunogen
Recombinant full-length human GATA-3 protein
Alternate Names
Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3; GATA binding protein-3; GATA-binding factor 3; GATA3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3

Database Links

Entrez Gene ID
2625
SwissProt
P23771

Additional Information

Clone
GATA3/4550R
Chromosome Location
10p14
Mol. Weight of Antigen
~50kDa

Related Antibodies

View All GATA3 Antibodies

Functions

  • Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity).

Key References

  • Higgins JP, et al. Am J Surg Pathol. 2007; 31:673-680.
  • Liu H, et al. Am J Clin Pathol. 2012; 138:57-64.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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