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GCHFR Antibody [GCHFR/7732]

In Stock
Catalog Number Formulation Size Price
2644-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2644-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2644-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
GCHFR Antibody

SDS-PAGE Analysis of Purified GCHFR Mouse Monoclonal Antibody (GCHFR/7732). Confirmation of Purity and Integrity of Antibody.

GCHFR Antibody - Image 2

Formalin-fixed, paraffin-embedded human liver stained with GCHFR Mouse Monoclonal Antibody (GCHFR/7732). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

GCHFR Antibody - Image 3

Western blot analysis of SK-BR3 cell lysate using GCHFR Mouse Monoclonal Antibody (GCHFR/7732).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

GTP cyclohydrolase I feedback regulatory protein (GFRP) is encoded by the gene GCHFR. GFRP mediates feedback inhibition of GTP cyclohydrolase I activity by tetrahydrobiopterin. GFRP also acts as a mediator for the stimulatory effect of phenylalanine on enzyme activity. L-phenylalanine reverses this inhibition. Cross-linking experiments have shown that GFRP is usually expressed as a homodimer or pentamer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Nuclear Marker
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Lysosome.
Gene Name
GCHFR
Positive Control
Human liver or intestine.
Immunogen
Recombinant fragment human GCHFR protein (exact sequence is proprietary)
Alternate Names
GTP cyclohydrolase 1 feedback regulatory protein, GTP cyclohydrolase I feedback regulatory protein, p35, GTP Cyclohydrolase I Feedback Regulatory Protein; GTP Cyclohydrolase I Feedback Regulator; GFRP; p35

Database Links

Entrez Gene ID
2644
SwissProt
P30047Human

Additional Information

Clone
GCHFR/7732
Chromosome Location
15q15.1
Mol. Weight of Antigen
63kDa

Related Antibodies

View All GCHFR Antibodies

Functions

  • Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine.

Key References

  • Li L, Rezvan A, Salerno JC, et al. Circ Res. 2010;106(2):328-336. doi:10.1161/CIRCRESAHA.109.210658

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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