Learn about our comprehensive antibody validation methods to ensure monospecificity. Antibody Validation>>
Flow Cytometric Analysis of PFA-fixed HeLa cells. GCM2 Mouse Monoclonal Antibody (PCRP-GCM2-1B3) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GCM2 Mouse Monoclonal Antibody (PCRP-GCM2-1B3) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using GCM2 Mouse Monoclonal Antibody (PCRP-GCM2-1B3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL).The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( L&H ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
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