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Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody [ASTRO/1974R]

In Stock
Catalog Number Formulation Size Price
2670-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2670-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2670-RBM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).

Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).

SDS-PAGE Analysis of Purified GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).

SDS-PAGE Analysis of Purified GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).

Flow Cytometric Analysis of T98G cells using GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of T98G cells using GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S Recombinant Rabbit Anti-GFAP (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).

Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S Recombinant Rabbit Anti-GFAP (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).

GFAP Antibody in Western Blot (WB)

GFAP Antibody in Western Blot (WB)

Western Blot Analysis of human brain tissue lysate using GFAP Recombinant Rabbit Monoclonal Antibody (ASTRO/1974R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Chicken, Cow, Dog, Human, Mouse, Pig, Rabbit, Rat
Research Areas
Cardiovascular, Neuroscience, Endothelial Cell Marker, Neural Stem Cells, Neuroinflammation, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm
Gene Name
GFAP
Positive Control
T98G cells (FACS). Human brain lysate (WB). Brain or Astrocytoma (IHC).
Immunogen
Recombinant human full-length GFAP protein
Alternate Names
Glial fibrillary acidic protein, Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Database Links

Entrez Gene ID
2670
SwissProt
P14136

Additional Information

Clone
ASTRO/1974R
Chromosome Location
17q21.31
Mol. Weight of Antigen
~50kDa

Related Antibodies

View All GFAP Antibodies

Functions

  • GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Key References

  • Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339.
  • Van Muijen GN et. al. 1987, Lab Invest, 57:359-369.
  • Debus E, et. al. 1983, Differentiation, 25(2):193-203.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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