Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Glycophorin A / CD235a (Erythrocyte Marker) Antibody [JC159]

In Stock
Catalog Number Formulation Size Price
2993-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2993-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2993-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Glycophorin A / CD235a (Erythrocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Glycophorin A Mouse Monoclonal Antibody (JC159).

Glycophorin A / CD235a (Erythrocyte Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified Glycophorin A Mouse Monoclonal Antibody (JC159). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
GYPA
Positive Control
Erythrocytes in any human tissues.
Immunogen
Formalin fixed membrane from a Hairy cell leukemia
Alternate Names
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, Blood group--MN locus; GPA; GPErik; GpMiIII; GPSAT; GYPA; MN sialoglycoprotein; MNS; PAS2; Sialoglycoprotein alpha

Database Links

Entrez Gene ID
2993; 2994
SwissProt
P02724

Additional Information

Clone
JC159
Chromosome Location
4q31.22
Mol. Weight of Antigen
39kDa

Related Antibodies

View All GYPA Antibodies

Functions

  • Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

Key References

  • Andersson, L.C., et al. 1979. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia. Int. J. Cancer 23: 717-720.
  • Liszka, K., et al., 1983. Glycophorin A expression in malignant hematopoiesis. Am. J. Hematol. 15: 219-226.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Glycophorin A / CD235a (Erythrocyte Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales