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Glycophorin A / CD235a (Erythrocyte Marker) Antibody [N/A]

In Stock
Catalog Number Formulation Size Price
2993-RBP6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2993-RBP6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2993-RBP6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Glycophorin A / CD235a (Erythrocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human Placenta stained with Glycophorin A Rabbit Polyclonal Antibody

Glycophorin A / CD235a (Erythrocyte Marker) Antibody - Image 2

Western Blot Analysis of K562 cell lysate usingGlycophorin A Rabbit Polyclonal Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Product Properties & Targets

Antibody Type
Rabbit Polyclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
GYPA
Positive Control
Erythrocytes in any human tissues. K562 cells.
Immunogen
Recombinant human glycophorin A protein
Alternate Names
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, Blood group--MN locus; GPA; GPErik; GpMiIII; GPSAT; GYPA; MN sialoglycoprotein; MNS; PAS2; Sialoglycoprotein alpha

Database Links

Entrez Gene ID
2993; 2994
SwissProt
P02724

Additional Information

Clone
N/A
Chromosome Location
4q31.22
Mol. Weight of Antigen
39kDa

Related Antibodies

View All GYPA Antibodies

Functions

  • Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

Key References

  • Andersson, L.C., et al. 1979. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia. Int. J. Cancer 23: 717-720.
  • Liszka, K., et al., 1983. Glycophorin A expression in malignant hematopoiesis. Am. J. Hematol. 15: 219-226.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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