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Recombinant Granzyme B (NK/T-Cell Lymphoma Marker) Antibody [GZMB/4539R]

In Stock
Catalog Number Formulation Size Price
3002-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3002-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3002-RBM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Granzyme B (NK/T-Cell Lymphoma Marker) Antibody

Formalin-fixed, paraffin-embedded human spleen stained with Granzyme B Recombinant Rabbit Monoclonal Antibody (GZMB/4539R).

Recombinant Granzyme B (NK/T-Cell Lymphoma Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with Granzyme B Recombinant Rabbit Monoclonal Antibody (GZMB/4539R).

Recombinant Granzyme B (NK/T-Cell Lymphoma Marker) Antibody - Image 3

Western Blot Analysis of human spleen tissue lysate using Granzyme B Recombinant Rabbit Monoclonal Antibody (GZMB/4539R).

Recombinant Granzyme B (NK/T-Cell Lymphoma Marker) Antibody - Image 4

SDS-PAGE Analysis of Purified GZMB Recombinant Rabbit Monoclonal Antibody (GZMB/4539R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin s Disease.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytolytic granule, Secreted
Gene Name
GZMB
Positive Control
A431 cells. Human tonsil, spleen or Hodgkin s Lymphoma tissue (IHC).
Immunogen
Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)
Alternate Names
Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, Cathepsin G-like 1; CCPI; CGL1; CSPB; CTLA-1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; GRB; Human lymphocyte protein (Hlp); Lymphocyte protease; SECT; T-cell serine protease 1-3E

Database Links

Entrez Gene ID
3002
SwissProt
P10144

Additional Information

Clone
GZMB/4539R
Chromosome Location
14q11.2
Mol. Weight of Antigen
29-32kDa

Related Antibodies

View All GZMB Antibodies

Functions

  • Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3262682, PubMed:3263427, PubMed:1985927). It cleaves after Asp (PubMed:8258716, PubMed:1985927). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:32188940, PubMed:31953257). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed:9852092).

Key References

  • Shresta, S., et al. 1995. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells. Proc. Natl. Acad. Sci. USA 92: 5679-5683.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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