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GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody [SPM249]

In Stock
Catalog Number Formulation Size Price
7184-RTM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7184-RTM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7184-RTM1X-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody

Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).

GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).

GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody - Image 3

Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Product Properties & Targets

Antibody Type
Rat Monoclonals
Host
Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus Laevis
Research Areas
Cardiovascular, Hypoxia, Immunology, Cytokine Signaling
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Endoplasmic reticulum lumen, Melanosome, Sarcoplasmic reticulum lumen
Gene Name
HSP90B1
Positive Control
HePG2. U20S cells. About 50% of breast tumors express grp94 protein.
Immunogen
Purified glucose regulated protein 94 (grp94) from chicken oviducts
Alternate Names
Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, Glucose Regulated Protein 94; 94kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

Database Links

Entrez Gene ID
7184
SwissProt
P14625

Additional Information

Clone
SPM249
Chromosome Location
12q23.3
Mol. Weight of Antigen
94kDa

Related Antibodies

View All HSP90B1 Antibodies

Functions

  • Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).

Key References

  • Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987).
  • Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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